FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708220
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC2208.6952423E-725.9171127
CTTTCGT3700.0076430118.23546145
CGTTGGA7850.017.2505631
AACGGGA7850.017.2505638
GACGTGT2902.126641E-417.2036022
ACGGGAT6254.1836756E-1117.1052959
CCGATCT3403.7604157E-516.7698978
AAACGGG10100.016.2302727
TTCCGAT3851.08772234E-414.8097786
TCTTCCG4905.7045334E-513.0907874
GAAACGG11550.012.9585576
GGAAACG11050.012.899925
CTTCCGA4756.4350304E-412.0037165
ACGTGTG6350.00718127568.9791573
CCGAGTA8951.6544863E-48.7596952
CGAGTAG9553.33694E-48.2093483
CCTTAAT15353.3153447E-77.89332251
CCCAAGT21102.0192601E-87.0934281
CCCAAAG50300.07.08469771
CCCGAGT12155.5773504E-47.0392161
Produced by FastQC (version 0.11.5)