FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708220
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT2354.1836756E-1133.367828
TCCGATC2207.4760464E-1032.4026347
TTCCGAT3002.0445441E-823.7619326
CTTCCGA3305.6134013E-821.6017555
TCTTCCG4351.0245803E-616.3875394
AACGGGA8159.094947E-1214.869438
AAACGGG9801.8189894E-1213.8207157
ACGGGAT6102.39371E-612.85481559
GAAACGG10254.5110937E-1011.823016
CGTTGGA8606.747905E-811.6046641
GGAAACG10801.0768417E-911.2209125
CGAGGTC6457.307918E-49.9468551
CAAAGCG5950.00419709749.5846441
CCCAAAT28650.07.46448041
TGGACAT20200.0011896077.288752145
CCATATA13804.1195533E-57.2318921
CCCGAGT12155.56508E-47.0405721
CCCAAGT21553.0140654E-86.94664341
CCCTAAG10300.0062881696.92095041
CCCAACT13602.6953898E-46.8140831
Produced by FastQC (version 0.11.5)