FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812949
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA8350.017.3428941
ACGGGAT6051.3256795E-814.8175599
AACGGGA7408.8039087E-1013.9781038
GAAACGG9551.8189894E-1112.997446
AAACGGG9452.0190782E-1012.4052597
GGAAACG10359.222276E-1011.326545
TATCAGT16350.0086087978.781393145
TCGTTGG6350.0094106768.6877039
GTCACGA3209.238154E-58.226142140-144
GTTGGAA23501.2732926E-117.6294672
CCCATAT11755.547476E-47.0425851
CCCTACA9950.0062050016.93051671
CCACAAT19103.4165168E-76.85976031
CCTCAAT15102.801132E-56.85019641
CCCATAA14209.278359E-56.79873941
AGTCACG3900.0066634776.1871843140-144
GGATGGT18105.1832496E-56.0957921
CCCGAGT12500.0081389956.0683611
AATATCC27702.7948772E-85.9747564
CCAAATA31602.0991138E-95.8920361
Produced by FastQC (version 0.11.5)