FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546792
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC1050.00897600226.7712881
TCCGATC2003.8318298E-421.0823887
CGAATTA1850.006044655618.9931414
CCGATCT2500.001717385816.8659118
CTTCCGA2600.002232148316.217225
TCTTCCG3255.638535E-415.1360734
TTCCGAT2800.003658159415.0588486
CGTTGGA8707.4287527E-912.1163141
GAAACGG10156.0954335E-911.0777736
GGAAACG10851.4715624E-911.0107725
AAAGCGC4650.00830491610.5789762
AAACGGG10057.023664E-79.78957
AACGGGA8207.435406E-59.4270848
CCCTAAA15053.3905962E-88.4049391
CCCAATA9850.00468108157.134481
CCATAAA21654.323374E-86.8164761
CCTTGAT11650.00320898236.63537261
CCAAAGT42000.06.5255012
CCTGAAA25101.5967089E-86.4395081
CCCATAA12100.00476959676.38860231
Produced by FastQC (version 0.11.5)