FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546792
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2150.042.4936562
CGATAGA1450.001470695624.2337821
ACGTGTG4601.0913936E-1121.3889483
TCCGATC2653.7474274E-721.2159927
CCGATCT3402.0418865E-618.6029938
TTCCGAT3101.9187682E-518.136256
CGTTGGA7400.017.094641
AGACGTG4651.3064891E-716.6248971
CGTGTGC5704.6929927E-916.0283094
CTTCCGA3801.0966752E-414.7953625
AACGGGA6752.7775968E-914.5761728
ACGGGAT5005.054493E-614.0555949
AAACGGG9301.1277734E-1012.8465127
AATTGGC8700.003429997211.910941145
GGAAACG9502.1573214E-911.836295
GAAACGG9652.759407E-911.6523066
TCTTCCG4858.623027E-411.5922424
CCTTGGA13404.6675268E-88.9158621
GTGCTCT13002.5869758E-68.1089967
CCCAAAA25150.08.1036221
Produced by FastQC (version 0.11.5)