FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585247
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC4305.456968E-1222.5119979
GACGTGT4505.4005795E-918.4383962
CGATCGG750.002045241916.432144140-144
ACGGGAT7951.6734703E-1013.9157739
TTCCGAT5551.7542587E-512.4583788
CCGATCT5151.2068455E-412.0834178
CTTCCGA6456.285005E-611.7919997
CGTTGGA12902.9831426E-1010.1839991
CGTGTGC8157.479859E-610.180714
AGACGTG7956.332476E-59.5670941
AACGGGA11707.136987E-89.4555888
TCTTCCG7353.041382E-49.4073464
CCCGAAT6000.00578359759.2191991
CCCAAAG77650.08.54838851
ACGTGTG10251.1133646E-48.0949073
AAACGGG13909.906216E-77.95902067
CCAAAGT72350.07.8366382
GGAAACG14501.8731171E-67.62968255
GAAACGG14802.547351E-67.4750266
CCCAAGT33700.07.18112661
Produced by FastQC (version 0.11.5)