FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2585247
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2200.005023825430.14913145
GATCGGG3209.957899E-425.909409145
CGTCGTG4209.4870484E-715.7924145
GCGTCGT4302.2814675E-715.425136145
TCCGATC4101.40934735E-515.1854386
CCGATCT4553.7648046E-513.683587
GACGTGT3750.001873177412.9132462
CGTTGGA13400.012.3901071
CTTCCGA5051.0003872E-412.3287714
TTCCGAT5852.993787E-511.8253165
AGCGTCG4452.587319E-511.178891145
ACGGGAT9755.0446033E-810.6427859
CGTGTGC7603.8435384E-510.012624
AAACGGG16301.8189894E-129.7613287
GAAACGG17250.09.624786
AACGGGA13951.2223609E-99.4221078
GGAAACG18051.8189894E-129.1981965
TCTTCCG7550.00402821978.2463973
ACGTGTG9050.00238716377.64398963
CCCAAAG71300.07.1797751
Produced by FastQC (version 0.11.5)