FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054005
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCG1305.2791776E-447.69798145
GATCGGG4353.4833857E-421.381853145
GACGTGT5550.020.1079392
CGTCGTG4756.304778E-419.581276145
TCCGATC6750.016.5331947
CGTTGGA15850.016.2821961
CGAATAC3000.006081020513.9498823
CCGATCT8554.620233E-1013.0525218
ACGGGAT10700.013.0372729
AACGGGA14750.012.7676898
CTTCCGA9501.8371793E-1012.4814735
TTCCGAT8959.513315E-1012.4691696
AGACGTG8556.4246706E-912.2367381
CGTGTGC11101.6370905E-1111.9390894
AAACGGG17650.011.8554237
CAAAGCG10458.967618E-1011.3467951
GAAACGG20100.010.7573726
TCTTCCG11052.2573658E-910.7306794
GGAAACG20350.010.62521655
CGACGAT950.00989098710.3333185-89
Produced by FastQC (version 0.11.5)