FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962698
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG1750.001485829638.65283145
GACGTGT2901.1618704E-519.2240492
TCCGATC2450.001587101717.0662489
TTCCGAT3104.197587E-415.7358148
CTTCCGA3550.001172589713.7411347
CGTTGGA9051.5099431E-811.5503621
AACGGGA7853.4810364E-711.5405528
AGACGTG5100.00140931810.9313211
ACGGGAT5853.6637805E-410.7211059
GAAACGG10851.9666913E-810.276456
AAACGGG10401.1650627E-710.0510357
CGTGTGC5650.0033014079.867214
GGAAACG11303.8957296E-79.250515
ACGTGTG6250.0075825238.9199593
CAAGCGA11350.00266585786.75382389
TCAAGCG11500.00305998746.665738
TTCCCTC28150.0033459696.6080546145
GCCAAAA25353.3669494E-96.59760241
TATCCAC18405.6509776E-56.0597556
CCCAAAA32555.275069E-105.9945961
Produced by FastQC (version 0.11.5)