FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962698
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT3353.6379788E-1227.0488132
CGTCGTG2409.167398E-520.799637145
GCGTCGT2451.16548115E-420.375154145
AGACGTG5604.141839E-916.1809861
ACGTGTG6652.5283953E-914.6742533
CGTGTGC6201.589433E-814.6150834
CCCGAGA4152.4855777E-413.4366671
TCCGATC3200.013.0692597
CCGATCT3308.275763E-512.673228
TTCCGAT4006.320988E-712.1979746
AGCGTCG2807.364124E-611.885507145
ACGGGAT7301.8159571E-611.4579799
CGTTGGA9804.8641596E-810.6687821
AAACGGG12151.0842996E-89.7526437
AACGGGA10108.408733E-79.6617628
GAAACGG12652.0942025E-89.3671636
CTTCCGA4555.7262805E-69.1915665
CGTTACC1300.0086819148.774398105-109
GATTTCG6400.0091914468.7128395
GAGCGTC4300.001968487.739399145
Produced by FastQC (version 0.11.5)