FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333594
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5450.074.011752
AGACGTG6350.063.5218931
CGTGTGC7350.054.879464
ACGTGTG7800.050.8217243
GTGTGCT14800.026.7844245
GTGCTCT15600.026.3024717
TGTGCTC16250.026.1063186
GCTCTTC17300.024.5218329
TCCGATC4800.019.41479110-14
TTCCGAT5450.016.84405310-14
TGCTCTT25650.016.8102258
CCGATCT4950.016.57852715-19
CTTCCGA5800.014.86835410-14
CGTTGGA6503.045534E-813.9091041
CGATCTA1801.7850107E-711.59091959
TCTTCCG7250.011.510982510-14
GCACTTG9800.005426476711.200478145
ACGGGAT4350.00546605811.1912349
GAAACGG6951.3498511E-511.0072056
AACGGGA5700.00361000059.7607758
Produced by FastQC (version 0.11.5)