FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333594
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5700.090.32752
AGACGTG6200.075.187611
CGACGTG801.4370016E-1069.5765841
ACGTGTG7700.067.76943
CGTGTGC7800.066.9005664
GTGCTCT14900.036.422647
GTGTGCT15650.034.677155
GATCGGG1850.002867640233.804974145
TGTGCTC16400.032.242816
GCTCTTC18000.029.7633199
TGCTCTT24400.022.5268468
TCCGATC5600.021.36995310-14
TTCCGAT7000.016.4995910-14
CGTTGGA6801.2732926E-1116.3709621
CCGATCT6700.016.19992315-19
CTTCCGA6750.016.07992210-14
TCTTCCG7750.013.64598810-14
TAGATCT9553.0806864E-413.097215145
GGAAACG9001.0768417E-912.3691715
GAAACGG7902.90147E-812.3300296
Produced by FastQC (version 0.11.5)