FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406287
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5150.049.1912162
AGACGTG7900.032.067691
CTCGTAT3200.00578189729.29315145
CGTGTGC9500.026.6668194
ACGTGTG10350.024.476793
ACACGTC9500.014.80075145
CGTTGGA10750.014.2636461
TTTCGTG3902.1415262E-413.6752917
GTGCTCT20800.013.1410997
AACGGGA12050.012.724838
ACGGGAT8951.4370016E-1012.6630139
GTGTGCT21900.012.4810455
TCCGATC4900.012.24496710-14
GGAAACG14600.011.8722135
TGTGCTC23050.011.8583466
CACACGT14653.6379788E-1211.197382145
AAACGGG14650.010.9215637
GAAACGG14950.010.7024026
CACTGAC23101.4190693E-410.1448145145
GCTCTTC26500.010.062959
Produced by FastQC (version 0.11.5)