FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2738357
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT9500.074.4118352
AGACGTG12400.056.4389761
CGTGTGC12650.055.88244
ACGTGTG13550.052.6923643
GTGCTCT28300.025.478827
GTGTGCT28200.025.3184935
TGTGCTC30350.023.5249276
GCTCTTC33150.021.5379059
TCCGATC8700.019.01350610-14
TTCCGAT9200.016.44339810-14
TGCTCTT47800.015.2326328
CCGATCT9500.014.73354215-19
CTTCCGA10850.013.55186810-14
CGTTGGA13800.012.8063841
TCTTCCG12050.011.732985510-14
CGTATAA5652.326042E-411.2605511
CCCGGAA4600.007338115510.7573621
AACGGGA13806.366463E-1110.2451078
AAACGGG16451.8189894E-129.8838817
CGATCTG4050.09.77459115-19
Produced by FastQC (version 0.11.5)