FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3251120
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT9500.073.369942
AGACGTG12700.055.4318581
CGTGTGC16250.042.8931964
ACGTGTG16850.041.365843
GATCGGG4102.0254447E-730.615112145
GTGCTCT33350.021.5269857
GTGTGCT35550.020.390865
TGTGCTC36550.019.4515676
GCTCTTC38850.018.8382289
TCCGATC9400.017.49951410-14
CGATCGG900.00949640117.433607145
TTCCGAT10450.015.07418810-14
CGTTGGA16600.014.27621
ACGGGTA2950.00546485114.1765659
CCGATCT10400.013.53816515-19
TGCTCTT57200.012.9167018
ACGGGAT12500.012.2674559
AACGGGT4656.4924447E-411.9916478
CGATCTA2450.011.9488215-19
AAACGGG18700.011.5547867
Produced by FastQC (version 0.11.5)