FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3251120
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT11800.086.882612
AGACGTG14250.066.071841
CGTGTGC18450.058.2132574
ACGTGTG20050.052.1764373
CGACGTG1850.049.0082441
GATCGGG6050.043.484985145
GCGTCGG2152.874065E-643.187546145
GTGCTCT36800.029.7542747
GTGTGCT37300.028.9814685
TGTGCTC40800.026.4953146
GCTCTTC42900.025.360919
TGCTCTT58600.019.1613358
TCCGATC13700.018.83556710-14
CGTTGGA17500.017.9337861
AGAGCGG8704.3425644E-717.787975145
ACGGGAT12900.016.7598279
AACGGGA17850.016.0192878
TTCCGAT14900.015.63355410-14
CTTCCGA16350.013.73522110-14
CCGATCT16050.013.20979415-19
Produced by FastQC (version 0.11.5)