FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905066
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7550.059.4039732
CGCCAAT4653.2014214E-1045.858936145
AGACGTG10450.041.637511
CGTGTGC12650.035.4545444
ACGTGTG14100.031.8085123
AGACCGG5350.002484017522.143723145
GCACACG19250.017.231842145
GTGCTCT28700.016.093667
GTGTGCT28150.015.6947065
TCCGATC7400.014.47357810-14
GCTCTTC33350.014.0504379
TGTGCTC33350.013.6489966
AGTCACG7200.013.163213145
GTCACGC6250.012.218571140-144
CGTATGC4000.00511185311.846891145
TTCCGAT8800.011.10600510-14
CCGATCT8650.010.83426915-19
CCCCTAC16000.0096491410.36603145
CGTCTGA11050.010.238453140-144
TGCTCTT47850.010.0725218
Produced by FastQC (version 0.11.5)