FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3762518
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT15450.088.416662
AGACGTG19450.067.724951
CGTGTGC23400.058.9733544
ACGTGTG23650.057.7605633
CGACGTG1751.8189894E-1243.8087771
GCGTCGG2851.8735955E-532.8616145
GTGTGCT44050.031.1692815
GTGCTCT46500.030.2764457
TGTGCTC49200.028.331626
GCTCTTC53200.027.1184719
TGCTCTT73350.019.7637678
TCCGATC17600.018.61194210-14
TTCCGAT18950.016.32977710-14
GCGTCGT4801.991725E-516.259644145
CCGATCT18700.015.0572715-19
CTTCCGA20400.014.69077810-14
ACGGGAT13250.014.2021599
CGTTGGA18900.012.9066271
TCTTCCG23200.012.73750410-14
CCCGATT3950.002617009412.3511511
Produced by FastQC (version 0.11.5)