FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309855
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT12450.087.311912
AGACGTG16300.066.6891561
CGTGTGC17500.061.3094834
ACGTGTG19600.054.7406163
GTGTGCT37650.029.434515
GCTCTTC37900.029.4265949
GTGCTCT40400.027.7803617
TGTGCTC40100.027.6361436
TGCTCTT60050.018.6898678
TCCGATC13000.018.569710-14
TTCCGAT14450.016.02241910-14
CCGATCT14600.015.08425515-19
CCCGTAA4702.5443878E-615.0184221
CTTCCGA15950.014.51560910-14
CCGTAAC3000.005618778514.1173162
TCTTCCG17350.013.01884910-14
ACGGGAT13050.012.981449
CGATCTA3700.012.9726715-19
AACGGGA15800.012.06226358
CGTTGGA17050.011.591931
Produced by FastQC (version 0.11.5)