FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309855
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT15600.091.874562
AGACGTG18000.072.5643161
CGTGTGC21050.068.758374
ACGTGTG22200.065.196563
CGACGTG2250.059.6204071
GTGTGCT39500.037.357095
GTGCTCT42400.035.135047
GCTCTTC43200.034.6478279
TGTGCTC43950.033.7352756
TGCTCTT63900.023.9763328
CGCCAAT2105.1622605E-420.1723181
TCCGATC16650.019.76039110-14
TTCCGAT18000.016.866310-14
CCGATCT18400.015.88570115-19
CTTCCGA19350.015.76255610-14
TCTTCCG21450.014.21936910-14
CGTTGGA15850.012.917921
ACGGGAT10203.6379788E-1112.4593739
AACGGGA14700.012.0073338
AAACGGG19400.011.2819417
Produced by FastQC (version 0.11.5)