FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670406
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT6700.055.4815032
AGACGTG9250.040.1866041
CGTGTGC10950.034.5881084
ACGTGTG12300.029.0811923
GTGTGCT25250.014.9995955
GTGCTCT25950.014.8652597
GCTCTTC28950.014.0516279
CCCGAAA3600.001241581613.637751
CGTTGGA15000.013.5598191
AAATCGC3700.001526506613.2691625
TGTGCTC29050.013.0375146
ACCGCTC3250.00995145712.9483687
AATTACG3250.00995145712.9483687
AACGGGT4353.5120835E-412.8987588
TCCGATC7200.012.6636257
CCGATCT7500.012.1570798
TTCCGAT7800.011.68956
CTTCCGA8750.011.221925
CGCGCGG1300.0032604539.882856130-134
GAAACGG19300.09.8119126
Produced by FastQC (version 0.11.5)