FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490669
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT6600.069.977242
AGACGTG10350.045.9552041
CGTGTGC10550.043.777234
ACGTGTG11700.039.4743423
GATCGGG2951.3734085E-534.38512145
TCCGCGC1205.455003E-428.7220047
GTGTGCT24500.019.132375
TGTGCTC26200.018.6802646
GTGCTCT24750.018.382087
GCTCTTC29550.016.5625369
TCCGATC6650.016.3780210-14
TTCCGAT7700.012.89133110-14
CGATCTA2152.0218067E-812.8247089
CCGATCT7750.012.274487515-19
CTTCCGA8850.011.37196510-14
TGCTCTT42550.011.3402978
CGTTGGA10951.9645086E-1011.331421
AACGGGA11705.2750693E-1111.1942178
TCTTCCG10450.010.15851910-14
ACGGGAT8309.637013E-69.9661889
Produced by FastQC (version 0.11.5)