FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2490669
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7200.068.005342
AGACGTG9150.051.2513121
CGTGTGC11200.043.1019744
ACGTGTG11700.042.4388663
GCGTCGG1950.002692736734.2441145
GTGCTCT24900.021.3259587
GTGTGCT26050.018.79615
TGTGCTC28250.017.8205686
GCTCTTC30950.016.934419
TCCGATC8250.016.38397610-14
TGCTCTT43000.012.5095968
TTCCGAT9650.012.14895110-14
GCGTCGT4207.99003E-411.924285145
CCGATCT8800.011.44161515-19
CTTCCGA10750.010.39258810-14
TCTTCCG11900.09.8518817
CGTTTCC15050.0079060378.873886145
CGATCTA3004.78758E-98.73533315-19
CGTCGTG4709.422365E-108.309562135-139
GATTTCG7600.00439658958.166695
Produced by FastQC (version 0.11.5)