FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569487
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT12350.098.4519652
AGACGTG14250.073.981251
ACGTGTG18550.065.925073
CGACGTG2150.065.378771
CGTGTGC18750.064.097344
GTGCTCT31900.038.7763757
GTGTGCT32700.037.612795
GTCGCCG1650.002288930835.398067145
TGTGCTC35550.035.3882146
GCTCTTC37800.032.7239769
CGTCGTG2852.955709E-530.740425145
GCGTCGT2707.7603944E-427.040188145
TGCTCTT53350.023.449358
GATCGGG3150.0019060623.177305145
TCCGATC14150.021.0598210-14
TTCCGAT15350.018.58929410-14
CTTCCGA16350.017.10844410-14
CCGATCT15500.016.32360315-19
TCTTCCG17250.015.80839810-14
CGTTGGA11450.013.5041
Produced by FastQC (version 0.11.5)