FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186642
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT6050.087.725592
AGACGTG7450.071.240251
ACGTGTG8500.063.272523
CGTGTGC8650.061.357214
GTGCTCT13850.039.342457
GTGTGCT15200.035.848225
TGTGCTC15400.035.382666
GCTCTTC15900.035.1601269
TGCTCTT22000.025.4111828
TCCGATC5900.020.86977210-14
TTCCGAT6100.019.02542910-14
CCGATCT6450.016.67647715-19
CTTCCGA7100.015.94711310-14
TCTTCCG7600.014.52551310-14
CGTTGGA5951.9639592E-613.0826641
AACGGGA5350.001846944810.581738
CGATCTA1751.1601442E-610.51370415-19
CCCTATT7954.906064E-59.7914281
CCCTAAC5850.00387502729.6773081
CCCTAAT7603.367868E-49.3112261
Produced by FastQC (version 0.11.5)