FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186642
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG605.456968E-12162.59702145
GACGTGT6400.0101.7837752
AGACGTG7300.083.415411
ACGTGTG8900.073.9883963
CGTGTGC9050.071.979684
CGACGTG701.950821E-550.5757871
GTGCTCT14350.045.888287
GTGTGCT16150.040.335375
TGTGCTC16350.040.2750366
GCTCTTC18300.035.983439
GATCGGG1600.002472719234.84222145
TGCTCTT23750.027.7261818
TCCGATC7100.021.34155810-14
TTCCGAT7750.018.45526910-14
CCGATCT8050.016.53608515-19
CTTCCGA8250.016.30686210-14
TCTTCCG8800.015.28768310-14
CGTCGTG950.00333662914.670408145
ATCCCGT3750.00157145613.217148
CGATCTA2251.8189894E-1212.58775215-19
Produced by FastQC (version 0.11.5)