FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163096
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7450.080.2479862
AGACGTG9750.061.3176881
CGTGTGC11150.054.2494134
ACGTGTG11300.053.529293
GTGTGCT22150.026.9908585
GTGCTCT24100.025.6824877
GCTCTTC24250.024.6535059
TGTGCTC25900.023.6260436
TCCGATC7000.019.29188510-14
TGCTCTT36700.017.2483658
TTCCGAT8150.015.87931210-14
CCGATCT8300.014.57544515-19
CTTCCGA8750.014.30814810-14
TCTTCCG9400.013.46840410-14
AACGGGA9855.2768155E-79.9968528
CGTTGGA9352.930532E-69.7791981
AAACGGG11856.672417E-89.4967097
CGATCTA2202.3781322E-68.95172715-19
CCATAAC10651.5277743E-58.5854921
ACGGGAT7850.0049270918.0638859
Produced by FastQC (version 0.11.5)