FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163096
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT9400.094.302532
CGTGTGC13100.068.7415544
AGACGTG11600.068.533221
ACGTGTG13250.067.432383
CGACGTG1650.046.9017871
GTGCTCT23750.037.9163937
GTGTGCT24950.036.092765
TGTGCTC26900.033.4763686
GCTCTTC27650.032.313899
GATCGGG2454.615824E-429.551514145
TGCTCTT39500.023.1540478
TCCGATC9600.020.95923610-14
TTCCGAT10200.018.62276810-14
CTTCCGA10750.017.2773110-14
CCGATCT10400.017.04699715-19
CGTTGGA10050.016.1006131
TCTTCCG12450.014.80514310-14
AATCCCG6957.4693246E-414.584415145
AGGGGCG6050.00501375414.360569145
CGATCTG4400.013.750751515-19
Produced by FastQC (version 0.11.5)