FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570847
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT7550.082.926322
AGACGTG11700.053.5122761
CGTGTGC12000.052.1744774
ACGTGTG13200.047.4313433
GTGCTCT25550.024.779987
GTGTGCT28750.022.2665445
TGTGCTC29750.020.8087046
GCTCTTC31250.020.4852249
TCCGATC8100.017.89087310-14
ACGGGAT9500.017.0315259
CGTTGGA14000.016.5819361
ACACGTC6856.398971E-414.885412145
TGCTCTT44950.014.7111798
TTCCGAT9350.014.59618810-14
CCGATCT9150.013.99263815-19
GAAACGG16250.013.8530676
AACGGGA13300.013.7521638
AAACGGG15350.013.7487187
CTTCCGA10700.012.62312110-14
TCTTCCG12000.011.02112510-14
Produced by FastQC (version 0.11.5)