FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570847
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT9850.080.006472
AGACGTG11550.062.747811
ACGTGTG14250.055.7964943
CGTGTGC15300.052.427214
CGACGTG1657.835661E-834.1153141
GATCGGG2853.1729283E-530.422886145
GTGCTCT28100.029.296987
TGTGCTC31350.026.2598136
GTGTGCT31650.026.0109065
GCTCTTC36050.023.0313879
TCCGATC9450.019.2101710-14
TGCTCTT44900.018.8052128
CGTTGGA14450.018.503721
AAACGGG15850.015.9814657
TTCCGAT10750.015.83981910-14
ACGGGAT10100.015.3265599
GAAACGG16600.015.259416
CTTCCGA11300.015.06885510-14
AACGGGA13750.014.8401628
GGAAACG17600.014.3923995
Produced by FastQC (version 0.11.5)