FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640986
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT2850.034.56152
AGACGTG4001.8189894E-1224.6250691
TCCGATC2602.8112481E-818.942367
CCGATCT3303.2519223E-517.0563248
CGTGTGC5603.6579877E-916.3329524
ACGTGTG5755.1913958E-915.9068773
TTCCGAT3351.6302438E-614.7015336
CGTTGGA9551.72804E-1012.5243431
TCTTCCG5201.12235175E-412.1772324
AGCGCTC4250.004215357411.5882667
CTTCCGA4650.00823313610.5914283
GAAACGG10751.6413105E-79.8173036
AACGGGA8701.3209703E-59.7044618
GGAAACG10701.6238137E-69.2056335
ACGGGAT6200.00656160069.0783659
AAACGGG10551.3503626E-58.6696247
CCACAAC13052.6894413E-68.087051
CCGAGTA8050.0061571887.86603742
CCCTAAC8250.0076709457.67534641
CCCTAAT9250.00250489527.6061991
Produced by FastQC (version 0.11.5)