FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1640986
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5200.052.782642
AGACGTG5300.046.4752851
CGTGTGC7400.038.992584
ACGTGTG7550.036.3536073
CGTCGTG1600.002206162335.6633145
GATCGGG2652.107202E-532.29884145
TGTCGTT2054.482682E-420.5981031
GTGCTCT17200.020.049227
GTGTGCT16300.018.1339155
GCTCTTC19000.017.7794159
TGTGCTC18850.016.4274886
CGTTGGA8450.015.8243821
AGCGTCG2050.00580355913.917385145
TCCGATC5450.013.68797610-14
CCGATCT6100.012.69090758
TGCTCTT27250.012.6549218
CGCCATT6203.3158085E-612.4862171
TTCCGAT5900.011.9282816
GAAACGG12056.184564E-1111.09676556
CTTCCGA6450.010.911145
Produced by FastQC (version 0.11.5)