FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260216
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT4200.024.5848332
TCCGATC3302.707202E-820.8598587
CGTGTGC5851.8189894E-1218.827364
CCGATCT4251.1579905E-616.1970658
AGACGTG6601.6189006E-1015.6448941
TTCCGAT4852.6707494E-715.6126376
ACGTCTG7900.004089118414.807516145
AACGGGT3307.4064825E-414.6019018
CTTCCGA5209.392636E-613.23798755
ACGTGTG7301.1184966E-813.2017173
AACCCGT4000.003154796312.0465687
GCACACG11450.003412496511.919296145
CGTTGGA8601.0957774E-711.2061111
TCTTCCG6306.702757E-510.9265934
ACACGTC5953.264904E-89.830199145
AAACGGG12001.0859367E-89.7519847
GAAGTCC16350.0043591779.539603145
ATTCGAT23359.973045E-48.349706145
GAAACGG12401.771883E-68.3271216
TCAGGCC21200.0038905178.276843145
Produced by FastQC (version 0.11.5)