FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126328
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT6050.025.3271872
AGACGTG9000.017.7993831
ACGTGTG11100.014.43193153
CGTGTGC11200.014.3030754
CGTCCGA900.00696172310.83440810-14
TCCGATC5100.09.5597713
AACGGGA14903.601599E-109.3489628
ACGGGAT11454.7560752E-79.1244239
TTCCGAT6456.548362E-118.638733
CGTTGGA14752.8090653E-88.4996321
GGAAACG20202.3646862E-118.2752195
GAAACGG19551.0186341E-108.1940896
AAACGGG19104.622052E-97.6578277
CCGATCT6754.5614797E-87.2229384
GATTTCG10000.00591656476.9649766
CCCAAAA51100.06.54244331
CTTCCGA8552.7121132E-96.51693631
GTGCTCT29002.1464075E-106.48463347
ATTTCGT12150.0054687656.305746
GTGTGCT29201.7043931E-96.2016915
Produced by FastQC (version 0.11.5)