FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126328
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT5550.035.143272
AGACGTG7850.023.0717661
CGTGTGC11200.019.2806114
ACGTGTG12500.016.7181553
TCCGATC6000.09.2878653
CCGATCT6950.09.0205874
CGTTGGA15003.9908628E-98.823471
CTTCCGA8750.08.7571291
GTGCTCT27150.08.2102667
TGTGCTC28600.07.79401166
TTCCGAT7400.07.5307012
GTGTGCT29650.07.28306445
GCGTCGT3254.4931316E-57.185571130-134
TCTTCCG9951.8189894E-127.00090273
GCTCTTC34100.06.945479
ACCTCGT13052.1587283E-46.9392095
TCGTGTA4301.3392801E-56.6866813135-139
AGATCGG9000.06.672316130-134
AAACGGG18156.6902467E-66.52453237
GCTAGTT14105.5485725E-46.4224592
Produced by FastQC (version 0.11.5)