FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156940
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2727417.378616031604434No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA78515.002548744743214No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGATGGG73534.685230024213075No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA69874.452019880208997No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCGAGGTT57013.6325984452657063No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA40822.6009940104498535No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGAAGGGT40692.592710590034408No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACGG38212.434688415955142No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT35502.262010959602396No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG35032.232063208869632No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGTGATGT34242.1817255001911557No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA28491.815343443354148No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGTAACAGGT25301.6120810500828344No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAAGAG23531.499299095195616No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAGTGG22311.42156238052759No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA20541.3087804256403721No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT19461.239964317573595No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT18841.2004587740537784No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG18071.1513954377469096No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA15690.9997451255256785No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT14700.936663693131133No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG14530.9258315279724736No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG14220.9060787562125652No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA13970.8901491015674781No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA11580.7378616031604435No Hit
GATGAACGCTAGCGGGAGGCCTAACACATGCAAGCTGAGCGGTAGAGATC11070.7053651076844654No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC10150.6467439785905441No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT9710.6187077864151905No Hit
AGTGAACGCTGGCGGTAGGCCTAACACATGCAAGTCGAACGGCAGCACAG9520.6066012488849242No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGAAAGGT9310.5932203389830508No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGAGATGT9270.5906715942398368No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGATGGG8290.5282273480310947No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG8290.5282273480310947No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG8130.5180323690582388No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG7600.48426150121065376No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT7410.4721549636803874No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG6960.4434815853192303No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA6470.41225946221485915No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA6410.40843634510003823No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT6370.40588760035682425No Hit
AGTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCGAGGTT6290.4007901108703964No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6100.38868357334013No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC5370.3421689817764751No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5030.3205046514591564No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA4750.3026634382566586No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG4430.28227348031094684No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4200.2676181980374665No Hit
AGTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGAAGGGT4110.26188352236523516No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG4080.2599719638078246No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA4080.2599719638078246No Hit
GATTAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGAAAGTA4020.2561488466930037No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3840.24467949534854086No Hit
AGTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGGTGATGT3710.23639607493309545No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT3650.2325729578182745No Hit
ATTGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3610.23002421307506052No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGAACATAC3430.2185548617305977No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTT3390.21600611698738373No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAATTC3300.2102714413151523No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3170.2019880208997069No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG2990.19051866955524405No Hit
AGTGAACGCTGGCGGCATGCTTGACACATGCAAGTCGAACGGCAGCACGG2920.1860583662546196No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2830.18032369058238817No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCACGG2730.17395182872435325No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2710.17267745635274626No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2680.1707658977953358No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC2640.16821715305212182No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAAGAG2620.16694278068051485No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGCAAATAC2600.16566840830890786No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2580.16439403593730087No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC2550.1624824773798904No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA2540.16184529119408692No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2540.16184529119408692No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT2510.15993373263667643No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2490.15865936026506947No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2440.155473429336052No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTT2430.1548362431502485No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGAGTGG2360.15037593984962408No Hit
AGTGAACGCTGGCGGCATGCCTGACACATGCAAGTCGAACGGTAACAGGT2210.14081814706257167No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2200.14018096087676818No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGATGATGGG1960.12488849241748438No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA1870.11915381674525297No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG1810.11533069963043201No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1740.11087039632980758No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGATGATGGG1720.10959602395820059No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1710.1089588377723971No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAAGAG1640.10449853447177265No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT1620.10322416210016568No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC1620.10322416210016568No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT1610.10258697591436217No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG1610.10258697591436217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG26100.0295.367586
AACGCTG130500.0295.254435
TGAACGC122800.0295.24733
CTAGCGA8150.0294.99119
CTAGCTA12450.0294.991069
CGCTGGC130550.0294.915077
CTGGCGG130500.0294.765059
ACGCTGG130750.0294.57696
CGCTAGC26250.0294.242377
ATTGAAC74750.0294.182161
GCTGGCG131750.0292.080328
ACGAACG30350.0288.068572
CGAACGC30700.0285.265443
GACGAAC28250.0284.912081
ATGAACG40150.0283.22922
AAACGCT3550.0282.887274
TAAACGC3600.0278.958283
ATTAACG800.0276.90712
GATAAAC3600.0274.855961
ATAAACG3600.0274.855962
Produced by FastQC (version 0.11.5)