FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196051
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA119666.1035138815920345No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA119166.078010313642879No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT114905.860719914716069No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG100755.138968941754952No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA93274.757435565235577No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCCC78564.007120596171404No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG64203.2746581246716415No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG63133.2200804892604475No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT62353.180294923259764No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT55812.8467082544848026No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGACTAAAGA50122.556477651223406No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCC45662.3289858251169337No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGGACGCAATGCTTC40802.0810911446511366No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT38591.968365374315867No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT37711.923479094725352No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT35381.804632468082285No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG33911.7296519783117656No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTATT29761.5179723643337704No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA27511.403206308562568No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA27401.3975955236137534No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG25031.2767086115347537No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT20681.0548275703770957No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCA17730.9043565194770748No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG16740.8538594549377458No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC16300.8314163151424884No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA16130.8227451020397754No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA15310.7809192506031594No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT15210.7758185370133281No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA12900.6579920530882271No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT12880.6569719103702608No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT12370.6309582710621215No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA11930.6085151312668642No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG11900.6069849171899149No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT11370.5799511351638094No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA10800.5508770677017715No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTT10270.523843285675666No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT9730.49629943229057744No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA9320.4753865065722695No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA9250.4718160070593876No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTA8810.4493728672641303No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT8720.4447822250332822No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTATTT8590.43815129736650155No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTT8490.43305058377667033No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA8390.4279498701868391No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG8250.4208088711610754No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG7980.40703694446853117No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT7220.3682715211858139No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAAGGTT6970.35551973721123586No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT6760.3448082386725903No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA6740.34378809595462406No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6700.3417478105186915No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACCTT6240.31828452800546797No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAGCGAAGCGCTTT6150.3136938857746199No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA6100.31114352897970426No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC5940.3029823872359743No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT5920.30196224451800807No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA5490.28002917608173383No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT5450.27798889064580135No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACCTA5250.2677874634661389No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATTAAGACG5200.2652371066712233No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTAGAC5110.2606464644403752No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG4600.234632825132236No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGACTG4500.22953211154240477No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT4460.2274918261064723No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4230.2157601848498605No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTT4190.213719899413928No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT3550.1810753324390082No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC3420.17444440477222764No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATTATT3420.17444440477222764No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA3420.17444440477222764No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGACTAAAGA3250.16577319166951457No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG3060.15608183584883525No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTA3010.15353147905391965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA2970.15149119361798716No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATAC2940.1499609795410378No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGCTGA2730.13924948100239223No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGACATAT2700.13771926692544287No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2590.13210848197662853No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT2500.12751783974578043No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCATGG2460.12547755430984794No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATACCT2420.12343726887391546No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2380.12139698343798298No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2270.11578619848916863No Hit
GATGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG2230.11374591305323616No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTG2230.11374591305323616No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAACAGGA2090.10660491402747245No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2090.10660491402747245No Hit
GATGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGGACGCAATGCTTC2080.10609484266848933No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT2060.10507469995052307No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT2040.10405455723255684No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATTGAA2030.10354448587357372No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCTTAGA2030.10354448587357372No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTA2030.10354448587357372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG37000.0295.44146
AAACGCT108.4218767E-4295.441384
ATAAACG108.4218767E-4295.441382
CGCTGGC158650.0295.162087
CTGCGAA108.4606017E-4294.98932295
ATGCGAA108.4606017E-4294.98932295
CTAGCGA7800.0294.989329
AACGCTG158850.0294.97645
CTGGCGG158400.0294.896189
TGAACGC149500.0294.84853
CGCTAGC37100.0294.645057
ACGCTGG159000.0294.605226
CTAGCTA28150.0294.465339
ATGAACG137800.0292.761382
GCTAGCG9100.0291.747658
GCTGGCG160200.0291.582738
GATGAAC138400.0291.385471
GACGAAC43800.0291.056981
ACGAACG46950.0290.721862
GAACGCT199750.0289.007484
Produced by FastQC (version 0.11.5)