FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153402
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA140509.158941865164731No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG116827.6152853287440845No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG110617.210466617123636No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA70964.6257545533956534No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA53553.4908280204951696No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT49343.2163857055318705No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA44762.9178237571869987No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC42932.7985293542457073No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT33802.203361103505821No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA31732.068421532965672No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG25091.6355718960639365No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC23181.511062437256359No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT23001.4993285615572156No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG18611.2131523708947731No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT18241.1890327375132006No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC17801.1603499302486278No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT17131.1166738373684828No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG15781.0286697696249072No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT15671.0214990678087639No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT15531.0123727200427635No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA14160.9230648883326164No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT13330.8689586837198994No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT13320.8683068017366137No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG13170.8585285719873273No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC12930.8428834043884695No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG12190.7946441376253243No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA12100.7887771997757526No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG11680.7613981564777512No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC10650.6942543121993194No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA10590.690343020299605No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA10360.6753497346840328No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG10230.6668752689013181No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG9280.6049464804891723No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA8960.5840862570240284No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT8460.551492157859741No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA8410.5482327479433123No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT8370.5456252200101694No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGGTACTT8210.5351951082775974No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA7690.5012972451467386No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG7640.49803783523030987No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT7470.48695584151445226No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG7030.4582730342498794No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA6680.43545716483487834No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA6600.43024210896859233No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT6330.41264129541987715No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA6090.3969961278210193No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT5820.37939531427230416No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG5490.35788320882387453No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA5420.35332003494087433No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA4940.3220296997431585No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG4810.3135552339604438No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA4810.3135552339604438No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT4590.2992138303281574No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT4510.2939987744618714No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTG4420.28813183661229974No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4290.27965737082958503No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC4110.26792349513044156No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA4090.2666197311638701No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA3900.25423397348144094No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3860.25162644554829794No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT3640.23728504191601152No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC3600.2346775139828685No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT3580.23337375001629704No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT3480.2268549301834396No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3380.22033611035058212No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT3340.21772858241743917No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3150.20534282473500995No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT2990.19491271300243804No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTA2920.1903495391194378No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA2880.18774201118629483No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2850.1857863652364376No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGCGAGGGCCC2640.17209684358743693No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2580.16818555168772245No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2560.16688178772115095No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG2530.16492614177129372No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2440.15905920392172201No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2440.15905920392172201No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2390.15579979400529329No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT2290.14928097417243583No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC2280.14862909218915007No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2230.14536968227272135No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2220.1447178002894356No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC2180.14211027235629262No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2160.1408065083897211No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA2160.1408065083897211No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2150.1401546264064354No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2110.1375470984732924No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA2020.13168016062372068No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA1970.12842075070729195No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA1950.12711698674072047No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1910.12450945880757747No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT1900.12385757682429172No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT1800.11733875699143426No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1790.11668687500814852No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT1700.1108199371585768No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG1690.11016805517529106No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA1680.10951617319200531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCT6800.0295.470124
ACGCTAG50600.0295.470126
AGATACG305.456968E-12294.9886295
CCAGACC350.0294.9886295
CTGGCAC108.45887E-4294.9886295
CAGACAC206.168739E-8294.9886295
CGCTGGC102550.0294.89397
CGCTAGC50700.0294.887337
TGAACGC99700.0294.87743
CTAGCTA40850.0294.627539
ACGCTGG102800.0294.176736
AACGCTG102900.0293.747285
TAAACGC6800.0293.297553
CTGGCGG103600.0291.856489
ATAAACG6850.0291.156682
GCTAGCT41350.0291.06498
GCTGGCG104300.0289.75638
GAACGCT149250.0289.234074
GCTAGCG10000.0289.088848
AACGCTA52050.0287.238985
Produced by FastQC (version 0.11.5)