FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108357
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1984418.313537657927036No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG79257.3137868342608225No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG73646.796053785173085No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCGATTCGATTTACCT48914.513783142759582No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA26472.442850946408631No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT24712.28042489179287No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG19641.8125271094622406No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA18301.6888618178797863No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA18161.675941563535351No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC16731.5439703941600451No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT15431.423996603818858No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT14741.3603182074069973No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG14191.309560065339572No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG13321.2292699133420082No Hit
GATGAACGCTGACAGAATGCTTAACACATGCAAGTCGATTCAATTTACCT13141.2126581577563056No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC12471.1508255119650785No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA12121.1185248761039897No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA11101.0243915944516737No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC11021.0170085919691392No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG9850.9090321806620708No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA9820.9062635547311203No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA9100.839816532388309No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGAATTTT8610.7945956421827848No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT7750.7152283654955379No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC7570.6986166099098351No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG7050.6506270937733604No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6760.6238637097741725No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA6470.5971003257749846No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT6200.5721826923964303No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA6110.5638768146035789No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT5930.547265059017876No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG5760.5315761787424901No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT5530.5103500466052031No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT5150.47528078481316394No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG5110.47158928357189667No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA5040.4651291563996789No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT4790.44205727364175823No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4710.43467427115922375No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT4500.41529388964257036No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4450.41067951309098627No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT4140.38207037847116476No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4140.38207037847116476No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3990.3682272488164124No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT3990.3682272488164124No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAACTTTCT3870.3571527450926105No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA3870.3571527450926105No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA3780.34884686729975917No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3620.33408086233469No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT3590.33131223640373947No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3530.3257749845418385No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3360.31008610426645256No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG3280.30270310178391796No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA3110.28701422150853195No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA3090.2851684708878983No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3030.2796312190259974No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2960.27317109185377964No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2940.271325341233146No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT2930.2704024659228291No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTGAACC2810.2593279621990273No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGTACC2610.24087045599269086No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2590.23902470537205717No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2550.2353332041307899No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2450.22610445102762164No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2440.22518157571730485No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTAA2380.21964432385540392No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT2340.2159528226141366No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA2250.20764694482128518No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT2210.2039554435800179No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2180.20118681764906746No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG2120.1956495657871665No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA2100.19380381516653286No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT2060.19011231392526556No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG2010.18549793737368145No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA1980.18272931144273097No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC1960.18088356082209733No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1870.17257768302924592No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT1860.1716548077189291No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA1830.16888618178797862No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA1810.167040431167345No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA1790.16519468054671135No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG1700.15688880275385994No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC1680.15504305213322628No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGTGGTCC1640.15135155089195898No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA1480.13658554592688982No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1440.13289404468562252No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA1430.1319711693753057No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGACCC1430.1319711693753057No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTGT1400.12920254344435522No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGTGGCCC1360.12551104220308795No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA1210.1116679125483356No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA1210.1116679125483356No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAACATGG1200.11074503723801878No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1200.11074503723801878No Hit
GACAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTACCCG1180.10889928661738511No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1140.10520778537611783No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGACGACCT1100.10151628413485055No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA1100.10151628413485055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC108.420276E-4295.399263
GATAAAC1800.0295.399261
AAACGCT1950.0295.399264
CGCTAGC35600.0295.399267
AACGCTG72350.0295.399265
ACGCTAG35600.0295.399266
TGAACGC84150.0295.223723
ACGCTGG65200.0294.040076
CGCTGGC65250.0293.814737
GCTAGCT15600.0293.099338
CTAGCTA15600.0293.099339
GCTAGCG20400.0292.82138
GAACGCT107100.0291.12414
ATTGAAC23150.0290.93321
ACGCTGA7600.0289.5696
GCTGACA7600.0289.168128
GACGAAC22000.0288.685641
ATGAACG60400.0287.818662
ATAAACG1850.0287.415472
CTAGCGG15000.0287.12399
Produced by FastQC (version 0.11.5)