FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117613
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA116709.922372526846521No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG105138.938637735624463No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG77296.571552464438454No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG76126.4720736653261115No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT59545.062365554828123No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA35843.047282188193482No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT33112.8151649902646816No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT32322.7479955447101934No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT30802.6187581304787733No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG30422.5864487769209186No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGGACGGCAGCGGGG26512.254002533733516No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT19571.6639317082295324No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA17931.5244913402430003No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT17561.4930322328314047No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC17091.4530706639572157No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA17011.4462686947871408No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG16181.3756982646476155No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA14671.2473110965624548No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAAATTGA14641.2447603581236768No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA13941.1852431278855229No Hit
GACGAACGCTGGCAGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA11350.9650293760043532No Hit
GATGAACGCTAGTGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG11100.9437732223478696No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTA10120.8604491000144541No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT9810.8340914694804146No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT9230.7847771929973728No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG8730.7422648856844056No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA7820.6648924863748056No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC7000.5951723023815395No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG6300.5356550721433855No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG6050.5143989184869019No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG5300.4506304575174513No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG4820.4098186424970029No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC4790.4072679040582249No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4630.3936639657180754No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCCC4530.38516150425548196No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA4430.37665904279288853No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA4290.36475559674525776No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC4130.35115165840510826No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC4100.34860091996633025No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3990.3392482123574775No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3960.3366974739186995No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3800.32309353557855No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3750.3188423048472533No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3550.30183738192206644No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT3380.2873831974356576No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT3250.27632999753428616No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3240.27547975138802683No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3040.25847482846284No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG2870.24402064397643117No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTATTTT2800.23806892095261578No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA2690.228716213343763No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2690.228716213343763No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT2650.22531522875872564No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTAGC2590.22021375188116962No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA2580.21936350573491026No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG2580.21936350573491026No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG2570.21851325958865092No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATCG2530.21511227500361352No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2410.20490932124850145No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT2280.19385612134713No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA2230.18960489061583327No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG2210.1879043983233146No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGAACATAC2210.1879043983233146No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2050.17430045998316512No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT2040.1734502138369058No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTG1870.15899602935049698No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCACCGCTTC1820.15474479861920026No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC1770.15049356788790355No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG1740.1479428294491255No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT1650.14029061413279145No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAAGGTT1620.13773987569401341No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT1620.13773987569401341No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA1600.13603938340149474No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT1580.13433889110897607No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA1550.131788152670198No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT1540.1309379065239387No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAGATGAG1520.12923741423142No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1510.12838716808516065No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA1480.1258364296463826No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA1360.11563347589127052No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGGGTTTTCT1350.11478322974501118No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACCTT1300.11053199901371448No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT1290.10968175286745513No Hit
GATGAACGCTAGGGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG1260.10713101442867709No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1230.10458027598989907No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA1210.10287978369738039No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC1210.10287978369738039No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTG1200.10202953755112106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAAAC1500.0295.490631
CGCTAGC48400.0295.490637
ATAAACG1450.0295.490632
TGAACGC76750.0295.298133
ACGCTAG49850.0295.194246
GGCTTGG108.456407E-4294.9881295
GGCCACT108.456407E-4294.9881295
CTAGTGA1050.0294.98819
GAGAACC108.456407E-4294.9881295
GCTAGCG22050.0294.98818
CTAGGGA350.0294.98819
CGCTGGC67600.0294.834937
ACGCTGG67600.0294.61646
CTGGCGG67000.0293.667249
CTAGCTA26650.0293.327769
AACGCTG67950.0292.881445
GACGAAC38400.0291.64311
GCTAGCT26950.0289.515238
ACGAACG39700.0288.791872
ATGAACG78050.0288.10812
Produced by FastQC (version 0.11.5)