FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117613
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2348719.969731237193166No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1360211.56504808141957No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT1289710.965624548306735No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT76396.495030311275114No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT41353.515767814782379No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT39093.323612185727768No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT32382.7530970215877497No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT25672.182581857447731No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT23271.978522782345489No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT22371.9020006291821483No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT19051.6197189086240467No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT18721.5916607857974885No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGCCTTCCTCTCAGAACCCCT16471.4003554028891365No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT14491.2320066659297868No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT11010.9361210070315356No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT10270.8732027922083443No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT9640.8196372849940058No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGCCTTCCTCTCAGAACCCCT9360.7958303928987442No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT9090.772873746949742No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGACCGTTCACCCTCTCAGGCCGGCT7770.6606412556435088No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT6320.5373555644359043No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT6190.5263023645345328No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT5870.49909448785423377No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT5790.4922925186841591No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5700.48464030336782493No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT5650.4803890726365283No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5420.4608334112725634No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5400.4591329189800447No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5330.45318119595622935No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT4810.40896839635074356No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGACCGTTCACCCTCTCAGGCCGGCT3800.32309353557855No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT3730.3171418125547346No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT3690.3137408279696972No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3660.3111900895309192No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT3280.2788807359730642No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT3140.2669772899254334No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3020.2567743361703213No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGAACCCCT2950.2508226131465059No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT2890.24572113626894987No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2810.23891916709887512No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2620.2227644903199476No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT2600.22106399802742896No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT2440.20746005968727946No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT2260.1921556290546113No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT2190.18620390603079592No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2000.17004922925186844No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT1920.1632472600817937No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGCGACCTTCCTCTCAGAACCCCT1840.15644529091171894No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTCCACCCTCTCAGGTCGGCT1840.15644529091171894No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT1820.15474479861920026No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1750.14879307559538488No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCAACCTCTCAGTCCGGCT1680.14284135257156946No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT1680.14284135257156946No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCGTCCTCTCAGACCAGCT1620.13773987569401341No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCGGACCTTCCTCTCAGAACCCCT1610.13688962954775408No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1590.1351891372552354No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT1560.13263839881645736No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT1550.131788152670198No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTTCGGCT1520.12923741423142No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1500.12753692193890132No Hit
CTGGACCGTGTTCCAGTTCNAGTGTGGCCGGTCATCCTCTCAGACCGGCT1470.12498618350012328No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1440.12243544506134525No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1350.11478322974501118No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT1260.10713101442867709No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT1230.10458027598989907No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1180.10032904525860235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)