FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170940
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2301913.466128466128465No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT170669.983619983619983No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT130867.6553176553176545No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT96425.640575640575641No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT80324.698724698724699No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT65953.858078858078858No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT49492.895167895167895No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT48242.822042822042822No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGAACCCCT44142.582192582192582No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT41372.4201474201474205No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT38432.248157248157248No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT34242.0030420030420033No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT31011.814086814086814No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT30631.791856791856792No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT28211.6502866502866502No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT26841.5701415701415704No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT26621.5572715572715572No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT23531.3765063765063765No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT23111.3519363519363519No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT17951.05007605007605No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT17501.0237510237510237No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT17491.0231660231660231No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT16780.9816309816309817No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT15080.8821808821808821No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT12610.7376857376857376No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT10230.5984555984555985No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT9410.5504855504855505No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT8690.5083655083655084No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT8350.48847548847548844No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCATCCTCTCAGACCGGCT8300.4855504855504855No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT8220.48087048087048084No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT7840.4586404586404586No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT7150.41827541827541825No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5390.3153153153153153No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCATCCTCTCAGACCGGCT5100.29835029835029836No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT4770.27904527904527904No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT4760.27846027846027843No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT4750.2778752778752779No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT4620.2702702702702703No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4550.26617526617526616No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4070.2380952380952381No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT3900.22815022815022815No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3600.2106002106002106No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3420.20007020007020004No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT3420.20007020007020004No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGATCCCCT3350.19597519597519597No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3230.18895518895518895No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT3060.17901017901017902No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT3010.1760851760851761No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2990.17491517491517491No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT2870.1678951678951679No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT2770.16204516204516203No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACTTCTCAGTCCGGCT2730.15970515970515972No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2590.15151515151515152No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGATCCCCT2590.15151515151515152No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2560.14976014976014976No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2420.14157014157014156No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2250.13162513162513162No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2140.1251901251901252No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2100.12285012285012285No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2050.11992511992511992No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCTGATCGTCCTCTCAGACCAGCT2030.11875511875511875No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2010.11758511758511757No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT1990.11641511641511643No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1940.1134901134901135No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTGAACCTCTCAGTCCCCCT1870.1093951093951094No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT1810.10588510588510587No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1780.10413010413010412No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1750.10237510237510238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCC59550.0292.33561
GACCGTA350.0292.335574
GTAGCTC950.0292.33557295
TGAGCTA206.4552296E-8292.33557295
CGTGTCT160200.0291.879367
GTGTCTC160250.0291.78838
CCGTGTC160850.0291.245126
GCCGTGT80300.0291.061375
TGTCTCA160600.0290.970379
TTGGACC42800.0290.96951
GGACCGT45200.0290.718663
ACCGTGT44100.0290.678345
GTCCGTG36600.0290.338754
TTGGTCC13700.0290.201721
CTGGTCC22950.0289.7881
GACCGTG44300.0289.03614
GGCCGTG80850.0288.5394
TGGACCG45500.0288.48062
TTGGGCC29600.0287.39751
CGTATCT9650.0286.27687
Produced by FastQC (version 0.11.5)