FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150607
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4292228.499339340136913No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT2338615.527830711719906No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT145599.666881353456347No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT83855.567470303505149No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT30342.014514597595065No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT27581.8312561833115328No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGGCCTTCCTCTCAGAACCCCT20681.373110147602701No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT19071.266209405937307No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT17861.1858678547477872No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT17451.1586446845100162No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT16991.1281016154627608No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT16481.094238647606021No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT15761.0464321047494471No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT12460.8273187833234843No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT11480.7622487666575922No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGGCCTTCCTCTCAGAACCCCT11280.7489691714196551No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT10740.7131142642772249No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT9510.6314447535639114No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT9150.6075414821356244No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8640.5736785142788848No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT8630.5730145345169879No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT8600.5710225952312974No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT7990.530519829755589No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT7360.488689104756087No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT6520.43291480475675104No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT6470.42959490594726674No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT6460.4289309261853698No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6430.4269389868996793No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT5690.37780448451931187No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5150.34194957737688153No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT5000.3319898809484287No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTAGAGTCTCTCAACTCGGCT4720.3133984476153167No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4540.30144681190117323No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCGCCCTCTCAGGCCGGCT4390.2914871154727204No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4060.2695757833301241No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT4050.26891180356822725No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCCCGGCT3990.2649279249968461No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT3990.2649279249968461No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3830.2543042488064964No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3810.25297628928270266No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3750.2489924107113215No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT3600.23903271428286865No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGCGACCTTCCTCTCAGAACCCCT3420.2270810785687252No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2990.19852994880716035No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT2940.19521004999767608No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2800.18591433333112006No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCGGACCTTCCTCTCAGAACCCCT2790.1852503535692232No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2770.1839223940454295No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2680.17794657618835777No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2590.17197075833128606No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2470.1640030011885238No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT2340.15537126428386464No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2320.15404330476007091No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACTCTCTCAAGTCGGCT2310.15337932499817405No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGGGGACCTTCCTCTCAGAACCCCT2270.15072340595058661No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAGCCTCTCAGCCCGGCT2180.14474758809351493No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2170.14408360833161807No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2090.1387717702364432No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT2080.13810779047454633No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT1970.1308040130936809No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1960.13014003333178403No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT1920.12748411428419662No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1870.12416421547471233No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1870.12416421547471233No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGACCTTCCTCTCAGAACCCCT1820.12084431666522805No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT1680.11154859999867205No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGACCTTCCTCTCAGAACCCCT1660.11022064047487833No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCGCTCAGAACCCCT1570.10424482261780661No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1550.10291686309401289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC82750.0292.185581
TGGACCG88450.0292.031682
GTGTCTC139800.0291.943948
GGACCGT88550.0291.866973
CGTGTCT139900.0291.839757
TGTCTCA139900.0291.73539
GACCGTG87650.0291.69514
ACCGTGT87650.0291.69515
CCGTGTC140250.0291.528386
GCCGTGT50500.0290.914865
CTGGGCC44750.0290.075561
CTGGACC5750.0289.819951
CGTATCT7400.0288.411387
GGCCGTG50950.0288.345464
TTGGGCC13150.0286.804021
CCGTATC7450.0286.475746
CTGGTCC2450.0286.395631
GTATCTC7550.0282.681378
GCCGTAT7450.0282.551425
GTCCGTG4400.0282.395324
Produced by FastQC (version 0.11.5)