FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104511
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATATTG69696.668197605993627No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG62826.010850532479835No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG60315.77068442556286No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT49824.766962329324186No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA49704.755480284371981No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG46474.446421907741769No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA44854.291414300886988No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG44444.2521839806336175No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT38883.72018256451474No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT33763.230281979887285No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT27782.658093406435686No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG25242.4150567882806593No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT19471.8629617934954217No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG13181.2611112705839578No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG12401.1864779783946189No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC12341.180736955918516No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATCATT11921.1405497985857949No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA11771.1261972423955373No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC10350.9903263771277664No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA8440.8075704949718212No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG7130.6822248375769058No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA6820.6525628881170403No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG6710.6420376802441848No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6640.6353398206887313No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA6440.6162030791017213No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA6060.5798432700864024No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA5950.5693180622135469No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC5780.5530518318645884No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT5390.515735185769919No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGCCCC5340.5109510003731664No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG5280.5052099778970635No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG5020.4803322138339505No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT4430.42387882615227107No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT4420.4229219890729206No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG4370.4181378036761681No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT4310.41239678120006507No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT4250.4066557587239621No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC4180.3999578991685086No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGCTTATTT4090.3913463654543541No Hit
GATGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGAGGTTATTA4070.38943269129565306No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGTGAAGGGA3860.3693391126292926No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGTCTT3780.3616844159944886No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3700.35402971935968464No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCGA3630.34733185980423115No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3610.3454181856455301No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3590.34350451148682914No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT3260.31192888786826267No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC3190.3052310283128092No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2830.2707848934561912No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATAA2620.2506913147898307No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2440.23346824736152177No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT2390.22868406196476926No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2380.22772722488541874No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT2370.22677038780606826No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT2280.21815885409191377No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA2280.21815885409191377No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2260.21624517993321274No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG2240.2143315057745118No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATAGAT2220.2124178316158108No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTAGC2210.2114609945364603No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAAGTATTT2200.2105041574571098No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG2200.2105041574571098No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA2180.2085904832984088No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATATTG2170.2076336462190583No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2140.2047631349810068No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA2060.19710843834620279No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC2020.19328109002880078No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG1980.1894537417113988No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA1920.1837127192352958No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT1890.1808422079972443No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA1880.1798853709178938No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT1830.17510118552114132No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1740.16648965180698683No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA1730.1655328147276363No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1700.16266230348958483No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT1670.15979179225153334No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT1650.15787811809283234No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA1620.15500760685478082No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1570.15022342145802833No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGAATATT1520.14543923606127585No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT1470.14065505066452336No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC1410.13491402818842035No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA1410.13491402818842035No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG1380.13204351695036887No Hit
GATTAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGAGAGATAC1350.12917300571231735No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG1350.12917300571231735No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG1290.12343198323621436No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC1290.12343198323621436No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC1280.12247514615686386No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG1280.12247514615686386No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1230.11769096076011137No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA1190.11386361244270937No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC1190.11386361244270937No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGATTTTTCA1190.11386361244270937No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1180.11290677536335889No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC1180.11290677536335889No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG1170.11194993828400837No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT1160.11099310120465788No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT1140.10907942704595688No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT1140.10907942704595688No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGTTTT1130.10812258996660637No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTACG1110.1062089158079054No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1080.1033384045698539No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1070.1023815674905034No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT1070.1023815674905034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC5300.0295.275883
GACAAAC108.4303954E-4295.275851
TGAACGC47800.0295.275853
AGGCACG157.2222356E-6294.99332295
CTGGCGG77500.0294.8039
ACGCTAG26750.0294.723946
CGCTGGC77800.0294.327037
GCTGGCG77700.0294.044168
AACGCTG77900.0293.94925
CGCTAGC26850.0293.626287
ACGCTGG78050.0293.573466
CTAGCTA16000.0293.149579
GACGAAC49200.0292.875241
AAACGCT5450.0292.566934
ATAAACG5350.0292.516272
GATAAAC5300.0292.490261
GCTAGCG11000.0292.311558
ATGAACG47850.0289.72212
GATGAAC48000.0289.124271
GAACGCT99950.0288.48114
Produced by FastQC (version 0.11.5)