FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96073
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA94179.801921455559835No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA88409.201336483715508No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG74967.802400258137042No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA62376.49193842182507No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT52575.471880757340772No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC40834.249893310295296No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA33403.4765230605893436No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCATCAT26452.7531148189397645No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA19632.0432379544721204No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA18171.8912701799673166No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG17861.8590030497642418No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC14991.5602718765938401No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT14741.534249997397812No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC13321.3864457235643728No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT12801.3323202148366347No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT11521.1990881933529711No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG10681.111654679254317No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCATTG9350.973218281931448No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA8460.8805803919935882No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA7720.8035556295733453No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT7300.7598388725240183No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC6750.7025907382927565No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA6640.6911411114465043No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG6590.6859367356072986No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT6070.6318112268795604No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAACACAG5260.5475003382844296No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC5020.5225193342562426No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATAT4950.5152332080813548No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA4390.456944198682252No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA4380.455903323514411No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4290.4465354470038408No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTAGAC4160.43300406982190626No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT4130.4298814443183829No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA4110.4277996939827006No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT3700.38512381210121466No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC3650.37991943626200914No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA3510.3653471839122334No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3500.3643063087443923No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATCA3450.3591019329051867No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG3380.35181580673029883No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA3340.3476523060589343No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3170.3299574282056353No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGTG3090.3216304268629063No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGA2990.3112216751844951No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT2980.310180800016654No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCATCGGGA2940.30601729934528954No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA2910.30289467384176616No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGCG2880.2997720483382428No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAACCA2800.2914450469955138No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT2650.27583191947789704No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAG2590.2695866684708503No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA2530.2633414174638036No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT2530.2633414174638036No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2420.25189179061755124No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2320.24148303893914003No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCGATTT2270.23627866309993442No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2260.2352377879320933No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG2230.23211516242856994No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG2180.22691078658936434No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2130.22170641075015873No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT1970.2050524080647008No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA1930.2008889073933363No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1900.19776628188981296No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTA1900.19776628188981296No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTACAC1730.18007140403651392No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTC1730.18007140403651392No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC1690.17590790336514942No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT1640.1707035275259438No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA1570.16341740135105595No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAAGTTTCAT1450.15092689933696252No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCATCAT1450.15092689933696252No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1340.13947727249071018No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA1330.13843639732286908No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT1290.13427289665150458No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1280.13323202148366348No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT1240.12906852081229897No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAGCGTA1240.12906852081229897No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACGG1190.12386414497309337No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1150.11970064430172889No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATATTT1130.11761889396604665No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1030.10721014228763544No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG980.10200576644842983No Hit
GACGAACGCTGGCGGCGTGCTTAACATATGCAAGTCGAACGAAGAGAGGT980.10200576644842983No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC970.10096489128058872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC206.108894E-8295.450473
ATAAACG206.108894E-8295.450472
ATTGAAC3700.0295.450471
TGAACGC68100.0295.233523
CGCTAGC52600.0295.169627
ACGCTAG52600.0295.169626
CGCTGGC43250.0295.108927
GTCCTGT108.453914E-4294.98907295
GCTAGCG23250.0294.989078
CAGACTC108.453914E-4294.98907295
ACGCTGG43300.0294.768136
CTAGCTA29250.0293.980569
CTGGCGG43500.0293.293739
AACGCTG43850.0291.070925
GACGAAC25900.0290.317171
GAACGCT97400.0287.867034
AACGCTA53950.0287.783545
CGAACGC28150.0282.331023
ACGAACG28400.0279.845672
CAGACAC950.0279.46332295
Produced by FastQC (version 0.11.5)