FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189712
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4784725.22086109471198No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2375812.52319305051868No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT104775.522581597368643No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA93294.917453824744877No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT70763.729864215231509No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA50752.675107531416041No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA45712.409441680020241No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC39782.096862612802564No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG31401.6551404233785947No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC28291.491207725394282No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAAATTTAC27361.4421860504343427No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA25191.3278021421944843No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC19661.036307666357426No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGCCCC19441.0247111410980856No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA16580.8739563127266594No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA13550.7142405330184701No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC12480.6578392510753142No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT12270.6467698406004893No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT11760.6198869865901999No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA8930.47071350257232014No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG8030.4232731719659273No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA7910.4169477945517416No Hit
ATTGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG7520.39639031795563806No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATATAT7480.39428185881757616No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC7340.38690225183435945No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG6980.3679261195918023No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT6790.35791093868600826No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA6410.3378805768744202No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5850.3083621489415535No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA5710.30098254195833685No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC5570.2936029349751202No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCAT5560.2930758201906047No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5400.2846419836383571No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAACCG5310.2798979505777178No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT5310.2798979505777178No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA5240.27620814708610947No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG4830.25459644092097494No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA4680.24668971915324278No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG4660.24563548958421183No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC4540.23931011217002612No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4510.2377287678164797No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGCTG4240.22349666863456186No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4200.22138820949649998No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT4170.21980686514295353No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGAGACAGTG4020.2119001433752214No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC3870.20399342160748926No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA3840.2024120772539428No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACATT3780.19924938854684995No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC3720.1960866998397571No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC3720.1960866998397571No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA3580.18870709285654044No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG3560.1876528632875095No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT3360.17711056759719998No Hit
GATGAACGCTGGCGGCGCAGTTAAGACATGCAAGTCGAACGAAAATTTAC3200.16867673104495234No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACT3140.1655140423378595No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT3110.16393269798431306No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3050.1607700092772202No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3030.15971577970818926No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATGT2970.1565530910010964No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT2800.1475921396643333No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGCAT2760.14548368052627142No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA2730.14390233617272497No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGTACGCTTCTTTTT2710.14284810660369401No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT2660.14021253268111664No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTAAAC2560.13494138483596188No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA2550.1344142700514464No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC2480.13072446655983805No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA2480.13072446655983805No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG2460.12967023699080713No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2400.12650754828371427No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC2390.1259804334991988No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA2380.12545331871468332No Hit
ACTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2360.12439908914565236No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGTGCC2350.12387197436113688No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA2210.11649236737792021No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2170.11438390823985832No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT2160.11385679345534284No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC2140.11280256388631188No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTAACAGGA2130.1122754491017964No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT2080.10963987517921903No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAAGG2070.10911276039470355No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG2020.10647718647212617No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGT2010.1059500716876107No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT2010.1059500716876107No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2010.1059500716876107No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT2000.10542295690309522No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAGGAAGG1940.10226026819600237No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT1930.10173315341148689No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1920.1012060386269714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC108.420399E-4295.456183
CGCTGGC172600.0295.370577
TGAACGC116200.0295.329043
ACGCTGG172800.0295.114236
CTGCGCC157.23194E-6294.98892295
CTGCGAA157.23194E-6294.98892295
GGACGCA108.460418E-4294.98892295
ATTGAAC54200.0294.911041
AACGCTG173100.0294.688085
CTGGCGG173000.0294.47749
GCTGGCG173350.0293.96798
CGCTAGC16700.0293.686987
GATAAAC12500.0293.092531
AGTGAAC5750.0292.8871
ACGCTAG16750.0292.810276
ATAAACG12550.0291.924842
GCTAGCT14350.0291.905438
GAACGCT177100.0290.868354
CGAACGC59250.0290.220253
GACGAAC59250.0288.724271
Produced by FastQC (version 0.11.5)