FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120622
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1618213.41546318250402No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA83596.929913282817396No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT57944.803435525857638No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA45543.775430684286449No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA44803.7140820082572No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT33742.7971680124687035No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG33172.7499129512029317No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG21551.7865729303112203No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG20721.717762928818955No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT19571.6224237701248527No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA16541.3712258128699573No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT16311.3521579811311368No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA15471.2825189434763145No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG15141.25516075011192No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT15001.243554243836116No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA14681.2170250866342789No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG13581.1258311087529638No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC12191.0105950821574836No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT12181.0097660459949263No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC11880.984894961118204No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA11740.9732884548424003No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT11530.9558786954286946No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATGTTT11530.9558786954286946No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT9900.8207458009318367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATCTTT9420.7809520651290809No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG8710.7220904975875048No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC8630.7154582082870455No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAGTGTC8300.6881000149226509No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG7890.6541095322577971No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGAGATAT7450.6176319411052711No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA7270.6027092901792377No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC6890.5712059160020561No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6770.561257482051367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA6280.520634710086054No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC6120.5073701314851354No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC6010.49825073369700384No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT6000.4974216975344464No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT5920.49078940823398715No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGAGA5800.4808409742832982No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA5730.4750377211453964No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG5730.4750377211453964No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA5080.42115037057916466No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT5050.41866326209149246No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT4870.40374061116545906No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC4780.3962792857024423No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT4750.3937921772147701No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT4670.38715988791431083No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC4510.37389530931339227No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA4500.37306627315083485No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT4430.367263020012933No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT4350.36063073071247365No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4240.35151133292434217No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA4230.35068229676178475No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG3990.3307854288604069No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGG3690.30591434398368456No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC3620.3001110908457827No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3610.2992820546832253No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3480.28850458456997896No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3370.2793851867818474No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3240.26860771666860106No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT3220.2669496443434863No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAAGCA3210.26612060818092886No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT3150.26114639120558436No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA3080.2553431380676825No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCATGG3020.25036892109233805No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTC3000.2487108487672232No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA2980.24705277644210838No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAAGG2920.24207855946676393No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCCATCC2870.2379333786539769No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA2850.2362753063288621No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGACGATTG2740.22715590854073056No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT2570.21306229377725458No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATGT2530.20974614912702494No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2370.19648157052610635No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2340.19399446203843412No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT2330.1931654258758767No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA2320.1923363897133193No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAGGAAGG2180.18072988343751556No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2140.17741373878728592No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGGAGAAGCCT2080.17243952181194144No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC2070.17161048564938403No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2070.17161048564938403No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTACAC2060.1707814494868266No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGAAACGACA2040.1691233771617118No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA2020.16746530483659697No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC2020.16746530483659697No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG2010.16663626867403958No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGG1980.16414916018636733No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1960.1624910878612525No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG1960.1624910878612525No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC1930.16000397937358027No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA1870.15502976239823582No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1870.15502976239823582No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC1850.153371690073121No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCCAACC1850.153371690073121No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC1850.153371690073121No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT1780.14756843693521912No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACCAAGG1760.1459103646101043No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT1740.14425229228498948No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA1720.14259421995987465No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCA1680.139278075309645No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA1680.139278075309645No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAA1680.139278075309645No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG1610.13347482217174314No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT1590.1318167498466283No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1570.1301586775215135No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACAGGA1550.12850060519639867No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT1510.12518446054616902No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGCGCGGTTAAAGCT1500.1243554243836116No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG1500.1243554243836116No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAATTTG1470.12186831589593937No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC1450.12021024357082455No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT1420.11772313508315232No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATTT1370.11357795427036528No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC1360.11274891810780788No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTATCC1360.11274891810780788No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC1310.1086037372950208No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1250.10362952031967634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC157.189359E-6295.358643
CGCTAGC12400.0295.358647
ACGCTGG107700.0295.221566
CAAAATC108.4564E-4294.9913295
AACGCTG107850.0294.947885
TGAACGC66450.0294.46973
ACGCTAG12500.0294.177226
CTAGCTA9050.0293.361489
CGCTGGC108500.0293.044777
GAACGCT116600.0292.572274
GACGAAC49750.0292.390231
AAACGCT4700.0292.216554
TAAACGC4550.0292.112983
ATAAACG4550.0292.112982
CTGGCGG108800.0292.008829
CGAACGC49950.0291.219483
ACGAACG49950.0291.219482
GCTGGCG109150.0291.20768
GCTAGCT9150.0290.155368
ATTGAAC1750.0286.919831
Produced by FastQC (version 0.11.5)