FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120783
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA117999.76875884851345No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG74766.1896127766324724No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT49324.083356101438116No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA41623.4458491675152962No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG30052.4879329044650325No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA29992.4829653179669324No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC24602.036710464220958No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT24592.0358825331379418No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA23801.9704759775796263No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC23251.9249397680137104No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT20941.7336876878368646No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA20611.706365962097315No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA20131.6666252701125157No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG17801.4737173277696365No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTAA16191.3404204234039558No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC15141.2534876596872075No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT12601.0431931646009787No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG11120.9206593643145143No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG10600.8776069479976487No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA10370.8585645330882657No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC10190.843661773593966No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT10090.8353824627637995No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG9710.8039210816091668No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT9610.7956417707790004No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT9290.7691479761224675No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA9170.7592128031262677No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC9130.7559010787942012No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA9060.7501055612130847No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA8710.7211279733075019No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA7920.6557214177491866No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG7890.6532376245001366No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC6560.5431227904589222No Hit
GAGAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAGTTTAGT6070.5025541673911064No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGGCCC5980.49510278764395654No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT5930.4909631322288732No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT5550.45950175107424057No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT5530.4578458889082072No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG5520.4570179578251906No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5520.4570179578251906No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT5500.45536209565915736No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA5420.44873864699502414No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG5410.44791071591200754No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG5370.44459899157994087No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA5000.4139655415083248No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG4960.4106538171762582No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC4910.40651416176117505No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4840.4007186441800584No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT4540.37588071168955894No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA4340.359322090029226No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT4240.35104277919905946No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT4220.34938691703302616No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4190.3469031237839762No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG3990.33034450212364325No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3990.33034450212364325No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA3860.3195813980444268No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA3780.3129579493802936No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT3760.3113020872142603No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT3650.3021948453010771No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG3570.29557139663694393No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT3490.28894794797281076No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC3470.28729208580677745No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA3450.28563622364074415No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC3340.27652898172756096No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT3260.2699055330634278No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3250.26907760198041114No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG3220.26659380873136124No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA3160.2616262222332613No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG3040.2516910492370615No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC2870.23761622082577846No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC2810.23264863432767854No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2800.23182070324466192No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG2650.21940173699941218No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA2540.21029449508622902No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2500.2069827707541624No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA2490.20615483967114578No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG2390.19787552884097925No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA2360.1953917355919293No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG2320.19208001125986274No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT2290.18959621801081278No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC2280.18876828692779613No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT2250.1862844936787462No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT2060.1705538031014298No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT2040.16889794093539653No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG2040.16889794093539653No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG2030.1680700098523799No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA1980.16393035443729664No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT1970.16310242335428No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGCTTATTT1950.16144656118824668No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT1940.16061863010523003No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTTATGG1930.1597906990222134No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCCCTT1910.1581348368561801No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA1880.15565104360713014No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT1870.1548231125241135No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC1820.15068345710903025No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGTAAGTCGTACGCGAAAGGGA1800.14902759494299694No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAG1790.1481996638599803No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGTGT1770.14654380169394698No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGCGA1710.14157621519584712No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTATGG1690.1399203530298138No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1680.13909242194679716No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT1670.13826449086378048No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA1670.13826449086378048No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT1660.13743655978076386No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1650.1366086286977472No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT1640.13578069761473055No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA1640.13578069761473055No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG1540.12750138678456405No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1490.1233617313694808No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA1470.12170586920344752No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAGCTTTCT1470.12170586920344752No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA1470.12170586920344752No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC1470.12170586920344752No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG1460.12087793812043085No Hit
GACGAACGCTGGCGGCGTGCTTAAGACATGCAAGTCGAACGGAGTTAAGG1450.1200500070374142No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAGGAT1420.11756621378836425No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG1420.11756621378836425No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA1390.11508242053931429No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG1390.11508242053931429No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT1330.11011483404121442No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA1320.10928690295819775No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT1320.10928690295819775No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCACA1300.10763104079216446No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT1270.10514724754311451No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC1260.10431931646009787No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG1260.10431931646009787No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG1250.1034913853770812No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC1240.10266345429406455No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1240.10266345429406455No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC1220.10100759212803127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGAAC700.0295.358221
ATAAACG6500.0295.358222
CTTGGTT108.4564113E-4294.9913295
CTGAACC108.4564113E-4294.9913295
TACTGCG108.4564113E-4294.9913295
ATTGGTT1400.0294.9913295
TGAACGC76550.0294.972353
ACGCTGG91400.0294.873476
CTAGCTA14300.0293.959879
CGCTAGC29200.0293.840947
CTGGCGG91700.0293.54379
AACGCTG91900.0293.429845
CGCTGGC91700.0293.425667
ACGCTAG29250.0293.338656
AAACGCT7000.0293.24854
TAAACGC6600.0293.120643
GACGAAC34050.0292.755951
ATTGAAC17900.0292.05811
CTAGCGA11450.0289.83869
ATGAACG58700.0288.56552
Produced by FastQC (version 0.11.5)