FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102237
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT88968.701350782984633No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT58885.759167424709254No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT54855.364985279302014No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT49274.819194616430451No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT48284.722360789146786No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT38363.7520662773751186No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT34473.371577804513043No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT29762.910883535315003No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT29092.845349530991715No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT27542.6937410135273923No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT25842.5274607040503927No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT24252.3719397087160226No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT22492.1997906824339526No Hit
TTGAACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT21762.128387961305594No Hit
ATGGGCCGTGTCTCAGTCCNATTGTGGGGGGCCTTCCTCTCAGAACCCCT20772.0315541340219294No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT19661.9229828731281238No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT16391.603137807251778No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT14911.458376126060037No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT13891.3586079403738371No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT13291.2999207723231316No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT12961.2676428298952434No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT12631.2353648874673553No Hit
TTGAACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT12601.23243052906482No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT11641.1385310601836907No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT11411.1160343124309202No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT8650.846073339397674No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT8220.8040142022946682No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT7700.7531519899840566No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT6650.6504494458953216No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT6620.6475150874927863No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGAACCCCT6520.6377338928176687No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT4870.47634418067822804No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4300.42059137103005767No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT4180.4088539374199165No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT3960.3873353091346577No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT3710.36288232244686364No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT3660.3579917251093048No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3190.312020110136252No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT3010.29441395972104034No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2990.29245772078601684No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT2860.2797421677083639No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2820.27582968983831685No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2730.267026614630711No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2690.263114136760664No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2560.25039858368301104No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2540.24844234474798751No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2450.23963926954038167No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGATCCCCT2450.23963926954038167No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2440.2386611500728699No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2410.2357267916703346No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2360.2308361943327758No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2350.22985807486526405No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2310.225945596995217No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2230.2181206412551229No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2150.21029568551502884No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT2100.20540508817747No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2060.20149261030742294No Hit
ATGGGCCGTGTCTCAGTCCCAGTGTGGGGGGCCTTCCTCTCAGAACCCCT1800.17606150415211713No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT1780.1741052652170936No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT1770.17312714574958185No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1750.17117090681455832No Hit
CTGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT1720.16823654841202304No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1680.164324070541976No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT1610.15747723426939367No Hit
CTGGGCCGTCTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1570.1535647563993466No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1540.15063039799681133No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCTACCTCTCAGTACGGCT1540.15063039799681133No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT1530.14965227852929958No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT1480.14476168119174077No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT1470.143783561724229No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1470.143783561724229No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1470.143783561724229No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT1460.14280544225671724No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1380.13498048651662314No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT1360.13302424758159961No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGTTCGTCCTCTCAGACCAGCT1330.13008988917906433No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1320.12911176971155258No Hit
ATGGGCCGTGTCTCAGTCCNATTGTGGGGGGCCTTCCTCTCAGATCCCCT1220.11933057503643495No Hit
ATGGGCCGTGTCTCAGTCCNAGTGTGGGGGGCCTTCCTCTCAGAACCCCT1210.11835245556892318No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT1180.1154180971663879No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1170.11443997769887615No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT1160.11346185823136437No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT1150.11248373876385262No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT1140.11150561929634085No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTTCGGCT1080.1056369024912703No Hit
TTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT1050.102702544088735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)