FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165922
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1866511.249261701281325No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA149669.01990091729849No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT90985.483299381637155No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT76584.61542170417425No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT47982.891720205879871No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT35342.1299164667735444No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA34512.079892961753113No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT33181.9997348151541088No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG30471.8364050577982425No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA25871.5591663552753705No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT24351.4675570448765083No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA21921.3211026868046432No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG20711.2481768541844964No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT20571.2397391545424958No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC20331.2252745265847809No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC19921.2005641204903508No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG18511.1155844312387748No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT18261.1005171104494882No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCTTTTT17901.0788201685129157No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT17121.0318101276503417No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA16631.0022781789033401No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG16150.97334892298791No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGCA15530.9359819674304795No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG15300.9221200323043358No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT14440.8702884487891902No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA13630.8214703294319017No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT13110.7901303021901858No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA12140.731669097527754No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC11920.7184098552331818No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACACT11160.6726052000337508No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG10670.6430732512867492No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT10520.6340328588131773No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG9950.599679367413604No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG9660.5822012752980316No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC9600.5785851183086028No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC9400.5665312616771736No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG9380.5653258760140307No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT9220.5556827907088873No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCATG7970.4803461867624546No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA7790.4694977157941683No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA7660.46166270898373934No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT7190.4333361458998807No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA7040.4242957534263087No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6550.39476380467930716No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA6030.3634237774375912No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA5970.3598076204481624No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA5930.3573968491218766No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA5780.34835645664830467No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT5250.3164137365750172No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT4970.29953833729101625No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGTGAAAGGA4790.28868986632272997No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACATTG4770.28748448065958704No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4630.27904678101758656No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG4520.2724171598703005No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCTATTT4520.2724171598703005No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG4420.2663902315545859No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4420.2663902315545859No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4300.2591579175757283No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT4230.25493906775472813No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA4170.25132291076529933No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT4100.24710406094429913No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA3820.2302286616602982No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3750.22600981183929797No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3720.2242017333445836No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA3530.21275056954472585No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA3160.19045093477658176No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT3050.18382131362929569No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2980.17960246380829545No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC2950.17779438531358108No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAATTTA2850.17176745699786647No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT2830.17056207133472354No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2810.16935668567158063No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2740.1651378358505804No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT2700.16272706452429456No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTTA2600.15670013620857995No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2530.1524812863875797No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG2520.15187859355600825No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA2500.15067320789286534No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC2490.15007051506129385No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGCCGTT2440.14705705090343654No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT2400.1446462795771507No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2390.14404358674557924No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2330.14042742975615047No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2330.14042742975615047No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTTAGA2190.13198973011415002No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA2170.13078434445100712No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCT2110.12716818746157835No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT2060.12415472330372102No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT2050.12355203047214956No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG1990.11993587348272079No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT1940.11692240932486349No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGGTTT1900.11451163799857764No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG1890.11390894516700618No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC1870.11270355950386327No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA1830.11029278817757743No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT1820.10969009534600596No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT1780.10727932401972011No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG1770.10667663118814864No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT1760.10607393835657718No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1750.10547124552500572No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT1710.10306047419871987No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC1670.10064970287243402No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA1670.10064970287243402No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT1670.10064970287243402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG25300.0295.434666
CGCTGGC140200.0295.223947
CGAACTT108.4594183E-4294.98944295
CGCTAGC25350.0294.851937
ACGCTGG140450.0294.803626
CTGGCGG140300.0294.674079
TGAACGC96600.0294.670073
AACGCTG141150.0293.13235
AAACGCT5700.0292.843144
TAAACGC5650.0292.82023
ATAAACG5600.0292.79682
GCTAGCG10400.0292.1538
GACGAAC61250.0291.575931
CTAGCTA15300.0291.133369
GATAAAC5650.0290.205721
GCTGGCG142650.0289.923038
ACGAACG62800.0288.84852
ATGAACG96050.0288.052642
GATGAAC96400.0287.00681
GAACGCT162550.0286.710664
Produced by FastQC (version 0.11.5)